Leadgene Leadgene
Human Galectin-3, His Tag, E. coli
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Catalog Number

LDG142PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 27 kDa.
      The protein migrates as 32 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to chemoattract human THP1 cells using a concentration range of 0.5-1 μg/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Galectin-3 (Gal-3) is one of the lectin family members, which is the only chimera‑type galectin, containing one carbohydrate recognition domain (CRD) connected to a long, flexible N‑terminal domain. The C‑terminal CRD is responsible for β-galactoside binding, and the N‑terminal domain is essential for its multimerization, and interaction with other intracellular proteins. Galectin-3 is predominantly presented in the cytoplasm and expressed on the cell surface, and then often secreted into biological fluids, such as serum and urine. Numerous studies have indicated that galectin-3 plays a crucial role in cell proliferation, apoptosis, gene expression, immune surveillance, inflammation, fibrosis, and host defense.

      Synonyms

      Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen

    • Uniprot ID

      P17931

      Sequence Note

      Ala2-Ile250

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Activity analysis of recombinant Human Galectin-3, His Tag, E. coli. Measure by its ability to chemoattract human THP1 cells using a concentration range of 0.5-1 μg/mL.

    Activity analysis of recombinant Human Galectin-3, His Tag, E. coli.
    Measure by its ability to chemoattract human THP1 cells using a concentration range of 0.5-1 μg/mL.

    SDS-PAGE analysis of recombinant human Galectin-3.

    SDS-PAGE analysis of recombinant human Galectin-3.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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