Species of Origin
His Tag (N-term)
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
>98% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 19.48 kDa.
The protein migrates as 21kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant human G-CSF is > 2 x 10⁷ IU/mg.
<0.1 EU per 1 μg of the protein by the LAL method.
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins and makes the bone marrow produce more white blood cells so it can reduce the risk of infection after some types of cancer treatment also is an established useful clinical agent for increasing neutrophilic granulocytes levels. G-CSF is a 18.67 kDa protein having 207 amino acid which secreted by monocytes, macrophages, fibroblasts, and endothelial cells, regulates cell growth, maturation, development of myeloid cells.
Granulocyte colony-stimulating factor
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Human G-CSF, His Tag, E. coli (LDG170PHE) induced NFS-60 cell proliferation, with the ED50 at 44 pg/mL.
SDS-PAGE analysis of recombinant human G-CSF.1/2
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.