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Leadgene Leadgene
Human FX, His Tag, CHO
Catalog Number

LDG030PHM

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  • Specifications
    • Species of Origin

      Human

      Expression System

      CHO

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16.97 kDa.
      The protein migrates as 15-20 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Factor X (FX), also known as Stuart factor, is a vitamin K-dependent serine protease essential for blood coagulation. Upon activation, FX is converted into its active form, Factor Xa (FXa), which plays a pivotal role in the coagulation cascade by catalyzing the conversion of prothrombin to thrombin. Thrombin, in turn, converts fibrinogen into fibrin, leading to the formation of a stable blood clot. Dysregulation of FX activity can result in bleeding disorders or thrombotic events. Understanding FX's role in hemostasis is crucial for developing therapies for coagulation disorders and managing thrombotic conditions.

      Synonyms

      Coagulation factor X, Stuart factor, Stuart-Prower factor

    • Uniprot ID

      P00742

      Sequence Note

      Ala41-Arg182

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human FX.

    SDS-PAGE analysis of recombinant human FX.

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  • Datasheet & Documents
    MSDS
    Manual
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