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Leadgene Leadgene
Human Flt-3 Ligand, His Tag, E. coli
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Catalog Number

LDG092PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 18.6 kDa.
      The protein migrates as 15-19 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in BaF3 cells transfected with mouse Flt-3. The ED₅₀ for this effect is <0.8 ng/mL. The specific activity of recombinant human Flt-3 Ligand is > 1 x 10⁶ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Fms-related tyrosine kinase-3 ligand (Flt-3 Ligand) is a protein which is encoded by the FLT3LG gene in human. As an important regulator of hematopoiesis, Flt-3 ligand has a tyrosine-protein kinase activity that stimulate the proliferation of hematopoietic progenitor cells of both lymphoid and myeloid origin. Flt-3 ligand can synergistically increase the proliferation of immature progenitors with other cytokines such as GM-CSF, G-CSF, IL-3, EPO, IL-11, IL-12, IL-6 or TPO. Flt-3 ligand is expressed by a variety of hematopoietic progenitor cells including immature hematopoietic and marrow stromal cells.

      Synonyms

      Fms-related tyrosine kinase 3 ligand, Flt3L, SL cytokine

    • Uniprot ID

      #P49771

      Sequence Note

      Thr27-Ala181

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human Flt-3 Ligand, His Tag, E. coli (LDG092PHE) induced proliferation in BaF3 cells transfected with mouse Flt-3.

    Human Flt-3 Ligand, His Tag, E. coli (LDG092PHE) induced proliferation in BaF3 cells transfected with mouse Flt-3.

    SDS-PAGE analysis of recombinant human Flt-3 Ligand.

    SDS-PAGE analysis of recombinant human Flt-3 Ligand.

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  • Datasheet & Documents
    MSDS
    Manual
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