Human FGF-8b, His Tag, E. coli

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Catalog Number

LDG074PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 22.14 kDa.
    The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is 1.4-3.8 ng/mL. The specific activity of recombinant human FGF-8b is > 2 x 10⁵ IU/mg.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Fibroblast Growth Factors-8b (FGF-8b) is a 21.3 kDa member of the fibroblast Growth Factors with 183 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation. FGF-8b having a more potent inductive activity due to higher affinity for Fgf receptors.

    Synonyms

    Androgen-induced Growth Factors, AIGF, Heparin-binding Growth Factors 8, HBGF-8, Fibroblast Growth Factors 8

  • Uniprot ID

    #P55075

    Sequence Note

    Gln52-Arg223

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human FGF-8b, His Tag, E. coli (LDG074PHE) induced 3T3 cell proliferation, with the ED50 at 3.51 ng/mL.

  SDS-PAGE analysis of recombinant human FGF-8b.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.