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Species of Origin
Human
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 22.14 kDa.
The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is 1.4-3.8 ng/mL. The specific activity of recombinant human FGF-8b is > 2 x 10⁵ IU/mg.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
More Images
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Specifications
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Background
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Background
Fibroblast Growth Factors-8b (FGF-8b) is a 21.3 kDa member of the fibroblast Growth Factors with 183 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation. FGF-8b having a more potent inductive activity due to higher affinity for Fgf receptors.
Synonyms
Androgen-induced Growth Factors, AIGF, Heparin-binding Growth Factors 8, HBGF-8, Fibroblast Growth Factors 8
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Uniprot ID
P55075-3
Sequence Note
Gln23 - Arg215
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Human FGF-8b, His Tag, E. coli (LDG074PHE) induced 3T3 cell proliferation, with the ED50 at 3.51 ng/mL.]()
Human FGF-8b, His Tag, E. coli (LDG074PHE) induced 3T3 cell proliferation, with the ED50 at 3.51 ng/mL.
![SDS-PAGE analysis of recombinant human FGF-8b.]()
SDS-PAGE analysis of recombinant human FGF-8b.
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Review
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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