Language
TW
Leadgene Leadgene
Human FGF-8a, His Tag, E. coli
More Images
Catalog Number

LDG073PHE

More Documents
Loading package & price...
  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 22.14 kDa.
      The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 μg/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Fibroblast Growth Factors-8a (FGF-8a) is a 23.5 kDa member of the fibroblast Growth Factors with 204 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation.

      Synonyms

      Androgen-induced Growth Factors, AIGF, Heparin-binding Growth Factors 8, HBGF-8, Fibroblast Growth Factors 8

    • Uniprot ID

      #P55075

      Sequence Note

      Gln52-Arg223

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human FGF-8a, His Tag, E. coli (LDG073PHE) induced 3T3 cell proliferation, with the ED50 at 1.93 μg/mL.

    Human FGF-8a, His Tag, E. coli (LDG073PHE) induced 3T3 cell proliferation, with the ED50 at 1.93 μg/mL.

    SDS-PAGE analysis of recombinant human FGF-8a.

    SDS-PAGE analysis of recombinant human FGF-8a.

    1
    /
    2
    Total   (2)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.