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Species of Origin
Human
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of 50 mM Tris, 1 M NaCl, pH 8.5.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 22.14 kDa.
The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 μg/mL.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Fibroblast Growth Factors-8a (FGF-8a) is a 23.5 kDa member of the fibroblast Growth Factors with 204 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation.
Synonyms
Androgen-induced Growth Factors, AIGF, Heparin-binding Growth Factors 8, HBGF-8, Fibroblast Growth Factors 8
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Uniprot ID
#P55075
Sequence Note
Gln52-Arg223
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
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Review
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.