Human FGF-11 Isoform 2, His Tag, E. coli

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Catalog Number

LDG138PHE

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 19.34 kDa.
    The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <1 ng/mL

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    The Fibroblast Growth Factors (FGF) family has two group, secreted FGFs and intracellular FGFs (iFGFs). iFGFs are related to voltage-gated sodium (Nav) channels. Human FGF-11 isoform 2 is a 18 kDa cytokine with 166 amino acid residues.

    Synonyms

    Fibroblast Growth Factors-11 Isoform 2, FGF-11 Isoform 2

  • Uniprot ID

    #Q92914

    Sequence Note

    Pro64-Pro225

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human FGF-11 isoform 2, His Tag, E. coli (LDG138PHE) induced 3T3 cell proliferation, with the ED50 at 0.84 ng/mL.

  SDS-PAGE analysis of recombinant human FGF-11 isoform 2.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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