Leadgene Leadgene
Human FGF-1, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16.77 kDa.
      The protein migrates as 18kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.3 ng/mL. The specific activity of recombinant human FGF-1 is > 1 x 10⁶ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      Fibroblast Growth Factors-1 (FGF-1) is a Growth Factors of mitogenic peptides which is a 15 kDa protein containing 139 amino acid residues. Fibroblast Growth Factors-1 is secreted by the macrophage which induce endothelial cell proliferation and angiogenesis. During wound injury, fibroblast Growth Factors-1 helps the tissue remodeling. When the fibroblast Growth Factors-1 binds with the FGFR on the cell membrane which is endocytosed by FGFR and induces the cell cycle.


      Acidic fibroblast Growth Factors , aFGF, Endothelial cell Growth Factors , ECGF, Heparin-binding Growth Factors 1 , HBGF-1, Fibroblast Growth Factors 1

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human FGF-1.

    SDS-PAGE analysis of recombinant human FGF-1.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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