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Leadgene Leadgene
Human FASL, His-SUMO Tag, E. coli
Catalog Number

LDG117PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His-SUMO Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 29.51 kDa.
      The protein migrates as 30-35 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce apoptosis in Jurkat cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human FasL is > 1 x 10⁶ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Fas Ligand (FasL) is a 17.34 kDa tumor necrosis factor with 152 amino acid residues. FasL is expressed from lymphoid tissue and secreted to blood. Binding to its receptor, TNFRSF6/FAS, leads to induce apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development.

      Synonyms

      Fas ligand, FasL, Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, CD178, Tumor necrosis factor ligand superfamily member 6

    • Uniprot ID

      #P48023

      Sequence Note

      Gln130-Leu281

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human FASL.

    SDS-PAGE analysis of recombinant human FASL.

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  • Datasheet & Documents
    MSDS
    Manual
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