Human EpCAM, His Tag, HEK293

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Catalog Number

LDG058PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    HEK293

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 28.25 kDa.
    The protein migrates as 30-35 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-3 μg/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Human epithelial Cell Adhesion Molecule (EpCAM) is a transmembrane glycoprotein involved in cell adhesion, signaling, migration, proliferation, and differentiation. It is widely used as a marker for epithelial cells and is overexpressed in various carcinomas.

    Synonyms

    Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326

  • Uniprot ID

    P16422

    Sequence Note

    Gln24-Lys265

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human EpCAM, His Tag, HEK293.
  Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-3 μg/mL.

  SDS-PAGE analysis of recombinant Human EpCAM.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.