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Species of Origin
Human
Expression System
CHO
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>90% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 58.83 kDa.
The protein migrates as 60 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
CD73 (NT5E) catalyzes AMP to adenosine, playing a critical role in immune regulation, inflammation, and cancer immune evasion.
Synonyms
5'-nucleotidase, 5'-NT, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase, CD73
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Uniprot ID
P21589
Sequence Note
Trp 27 - Ser 549
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
"This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles."
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.