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Leadgene Leadgene
Human CD40L, His Tag, E. coli
Catalog Number

LDG116PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16.99 kDa.
      The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <5 ng/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Human CD40L (CD154) is one member of TNF family which is expressed on T cells. It binds the CD40, which is on the antigen-presenting cells (ex: B cells, macrophages and monocytes) Human CD40L is a 21.6 kDa cytokine with 103 amino acid residues which is a transmembrane glycoprotein. Human CD40L plays an important role in the adaptive immune system which induces the B cell maturation and activation.

      Synonyms

      CD40 ligand, CD40L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5

    • Uniprot ID

      #P29965

      Sequence Note

      Met1-Leu261

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human CD40L.

    SDS-PAGE analysis of recombinant human CD40L.

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  • Datasheet & Documents
    MSDS
    Manual
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