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Species of Origin
Human
Expression System
CHO
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 41.52 kDa.
The protein migrates as 45-47 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells, with the ED50 ranging from 1 to 5 μg/mL.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
More Images
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Specifications
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Background
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Background
CD4 is a glycoprotein expressed on the surface of T helper cells, crucial for immune system regulation. It facilitates interactions between T cells and antigen-presenting cells, pivotal in initiating and coordinating immune responses. Our product targets CD4 to modulate these interactions, potentially influencing immune function. Through extensive research, we have developed a solution designed to interact selectively with CD4, aiming to optimize immune response modulation for therapeutic benefits.
Synonyms
T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, CD4 antigen, T-helper antigen, L3T4, T4 antigen, HIV receptor
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Uniprot ID
P01730
Sequence Note
Met1-Trp390
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![HPLC analysis of recombinant Human CD4, His Tag, CHO. The purity of Human CD4 Protein is greater than 90% as determined by SEC-HPLC.]()
HPLC analysis of recombinant Human CD4, His Tag, CHO.
The purity of Human CD4 Protein is greater than 90% as determined by SEC-HPLC.![SDS-PAGE analysis of recombinant human CD4 protein.]()
SDS-PAGE analysis of recombinant human CD4 protein.
![Human CD4, His Tag, CHO supports the adhesion of NIH-3T3 mouse embryonic fibroblast cells, with the ED50 ranging from 1 to 5 μg/mL.]()
Human CD4, His Tag, CHO supports the adhesion of NIH-3T3 mouse embryonic fibroblast cells, with the ED50 ranging from 1 to 5 μg/mL.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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