Leadgene Leadgene
Human ApoJ, His Tag, HEK293
Catalog Number

LDG077PHM

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  • Specifications
    • Species of Origin

      Human

      Expression System

      HEK293

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 47.79 kDa.
      The protein migrates as 50-70 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Apolipoprotein J, also known as clusterin, is a multifunctional protein involved in lipid transport, immune response, and cellular processes. It plays a crucial role in the metabolism of lipoproteins and is found in various tissues, including the brain and liver. Apolipoprotein J is known for its involvement in neurodegenerative diseases, such as Alzheimer's, where it may contribute to the clearance of amyloid-beta plaques. Additionally, it acts as a chaperone, protecting cells from stress and promoting tissue repair. Its diverse functions make it a significant biomarker for various diseases and a potential target for therapeutic interventions.

      Synonyms

      Clusterin, CLU, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Sulfated glycoprotein 2, Testosterone-repressed prostate message 2

    • Uniprot ID

      P10909

      Sequence Note

      Asp23-Glu449, with a deletion of Arg214-Leu238.

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant Human ApoJ.

    SDS-PAGE analysis of recombinant Human ApoJ.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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