Leadgene Leadgene
Hot Start Taq DNA Polymerase (Chemical Modified)
Catalog Number

LDG0037RF

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  • Overview

    Description

    Hot Start Taq DNA Polymerase (Chemical Modified), a modified form of Taq enzyme, remains inactive at room temperature but activates at 95°C, preventing nonspecific amplification during PCR. Its rapid 5-minute activation time streamlines procedures. With an optimized buffer system, it significantly reduces primer-dimer formation, enhancing sensitivity and specificity. This enzyme possesses 5'-3' exonuclease activity, are applicable for fluorescence quantitative PCR reactions. Employing hot-start amplification is a common method to enhance PCR specificity, making hot-start enzymes an excellent choice.

  • Specifications
    • Application

      RT-PCR, qPCR

      Concentration

      5 U/µL

    • Unit Definition

      One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 minutes at 72°C by using activated salmon sperm DNA as a template/primer.

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.

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  • Datasheet & Documents
    MSDS
    Manual
    1
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    1

Disclaimer:For Research Use or Further Manufacturing Only.

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