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Leadgene Leadgene
HIV-1 Reverse Transcriptase
Catalog Number

LDG0022RF

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  • Overview

    Description

    The human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RTase is an enzyme that can catalyze complementary DNA (cDNA) synthesis from an RNA template. Due to its greater thermostability than comparatives of AMV and MMLV, HIV-1 RTase is currently used for RT-LAMP reactions, in combination with Bst DNA polymerase LF.

    Product Note

    10X Isothermal Amplification Buffer: 200 mM Tris-HCl (pH 8.8), 100 mM (NH₄)₂SO₄, 500 mM KCl, 20 mM MgSO₄, and 1% Tween 20.

    Components

    Package

    Items

    Quantity

    200 U

    HIV-1 Reverse Transcriptase (5 U/μL)

    1 vial (200 U)

    10× Isothermal Amplification Buffer

    1 vial (1 mL)

    100 mM MgSO₄

    1 vial (0.4 mL)

    • 200 U

      Items

      HIV-1 Reverse Transcriptase (5 U/μL)

      Quantity

      1 vial (200 U)

      Items

      10× Isothermal Amplification Buffer

      Quantity

      1 vial (1 mL)

      Items

      100 mM MgSO₄

      Quantity

      1 vial (0.4 mL)

  • Specifications
    • Expression System

      Escherichia coli

      Application

      Loop-mediated Isothermal Amplification (LAMP), Reverse transcription

    • Concentration

      5 U/µL

      Storage Buffer

      HIV-1 Reverse Transcriptase is supplied in 10 mM Tris-HCl (pH 7.4), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol (v/v).

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Unit Definition

      One unit is defined as the amount of the enzyme incorporates 1 nmol of dTTP into acidinsoluble product in 10 minutes at 50°C.

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in therapeutic procedures.

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