HIV-1 Reverse Transcriptase

Catalog Number

LDG0022RF

Datasheet

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• Overview

  Description

  The human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RTase is an enzyme that can catalyze complementary DNA (cDNA) synthesis from an RNA template. Due to its greater thermostability than comparatives of AMV and MMLV, HIV-1 RTase is currently used for RT-LAMP reactions, in combination with Bst DNA polymerase LF.

  Product Note

  10X Isothermal Amplification Buffer: 200 mM Tris-HCl (pH 8.8), 100 mM (NH₄)₂SO₄, 500 mM KCl, 20 mM MgSO₄, and 1% Tween 20.

  Components

  Package	Items	Quantity
  200 U	HIV-1 Reverse Transcriptase (5 U/μL)	1 vial (200 U)
  	10× Isothermal Amplification Buffer	1 vial (1 mL)
  	100 mM MgSO₄	1 vial (0.4 mL)

  • 200 U

    Items

    HIV-1 Reverse Transcriptase (5 U/μL)

    Quantity

    1 vial (200 U)

    Items

    10× Isothermal Amplification Buffer

    Quantity

    1 vial (1 mL)

    Items

    100 mM MgSO₄

    Quantity

    1 vial (0.4 mL)

• Specifications

  • Expression System

    Escherichia coli

    Application

    Loop-mediated Isothermal Amplification (LAMP), Reverse transcription

  • Concentration

    5 U/µL

    Storage Buffer

    HIV-1 Reverse Transcriptase is supplied in 10 mM Tris-HCl (pH 7.4), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol (v/v).

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit is defined as the amount of the enzyme incorporates 1 nmol of dTTP into acidinsoluble product in 10 minutes at 50°C.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use Only. Not for use in therapeutic procedures.