The human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RTase is an enzyme that can catalyze complementary DNA (cDNA) synthesis from an RNA template. Due to its greater thermostability than comparatives of AMV and MMLV, HIV-1 RTase is currently used for RT-LAMP reactions, in combination with Bst DNA polymerase LF.
10X Isothermal Amplification Buffer: 200 mM Tris-HCl (pH 8.8), 100 mM (NH₄)₂SO₄, 500 mM KCl, 20 mM MgSO₄, and 1% Tween 20.
HIV-1 Reverse Transcriptase (5 U/μL)
1 vial (200 U)
10× Isothermal Amplification Buffer
1 vial (1 mL)
100 mM MgSO₄
1 vial (0.4 mL)