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Leadgene Leadgene
Enterokinase ELISA Kit
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Catalog Number

LDG00026E

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  • Overview

    Description

    Enterokinase is a highly specific serine protease that cleaves fusion proteins by recognizing the -(Asp)4 Lys sequence. It is commonly used to release the native protein from the N-terminal fusion peptide or protein.
    Leadgene® Enterokinase ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Enterokinase level in sample solution. The Enterokinase ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.

    Components

    Enterokinase (EK) ELISA plate

    96 wells (12 x 8-well strips)

    Standard Enterokinase (EK)

    2 vials (Lyophilized form)

    Standard reconstitution buffer

    1 vial (1.1 mL)

    Standard & Sample diluent buffer

    1 vial (12 mL)

    HRP-antibody conjugate

    1 vial (70 μL)

    HRP-antibody conjugated diluent buffer

    1 vial (12 mL)

    20 X wash buffer

    1 vial (15 mL)

    TMB

    1 vial (12 mL)

    Stop solution

    1 vial (6 mL)

    Microplate sealing film

    1 sheet

    • Standard Enterokinase (EK)

      96 wells (12 x 8-well strips)

      2 vials (Lyophilized form)

    • Standard reconstitution buffer

      96 wells (12 x 8-well strips)

      1 vial (1.1 mL)

    • Standard & Sample diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • HRP-antibody conjugate

      96 wells (12 x 8-well strips)

      1 vial (70 μL)

    • HRP-antibody conjugated diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • 20 X wash buffer

      96 wells (12 x 8-well strips)

      1 vial (15 mL)

    • TMB

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • Stop solution

      96 wells (12 x 8-well strips)

      1 vial (6 mL)

    • Microplate sealing film

      96 wells (12 x 8-well strips)

      1 sheet

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      Limit of detection (LoD): 0.007 ng/mL.
      Limit of quantification (LoQ): 0.032 ng/mL.

      Specificity

      Enterokinase

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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