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Leadgene Leadgene
Enterokinase ELISA Kit
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Catalog Number

LDG00026E

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  • Overview

    Description

    Enterokinase is a highly specific serine protease that cleaves fusion proteins by recognizing the -(Asp)4 Lys sequence. It is commonly used to release the native protein from the N-terminal fusion peptide or protein.
    Leadgene® Enterokinase ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Enterokinase level in sample solution. The Enterokinase ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.

    Product Note

    • Leadgene Enterokinase ELISA Kit is designed for commonly used mutant enterokinase. We have incorporated three modifications (P92R, E185D, and C122S) on the antigen used for ELISA antibody production.
    • These alterations are employed to facilitate cleavage processes. However, they may cause conformational differences in the enterokinase protein.

    Test Principle

    Enterokinase ELISA kit is used to detect enterokinase in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-nuclease monoclonal antibody to the solid phase. Enterokinase in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-enterokinase monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Application

      Sandwich ELISA analysis

      Sensitivity

      Limit of detection (LoD): 0.007 ng/mL
      Limit of quantification (LoQ): 0.032 ng/mL

    • Specificity

      Enterokinase

      Assay Range

      0.3125 ng/mL-20 ng/mL

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    Enterokinase cleaves fusion proteins by recognizing the -(Asp)4 Lys sequence.

    Enterokinase cleaves fusion proteins by recognizing the -(Asp)4 Lys sequence.

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

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  • Datasheet & Documents
    Datasheet
    MSDS
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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