Description
Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.
Product Note
- After thawing, the buffers may crystallize, which is a normal occurrence. Warm crystallized buffer until the salt crystals return to solution. Ensure that your components return to RT before use in the assay.
- Please fine-tune the input sample volume to find the optimal condition for your assay.
- Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein.
Components
10,000 U
Items
Endoglycosidase H (Endo H) (500 U/μL)
Quantity
1 vial (10,000 U)
Items
10× Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
Items
10× Reaction buffer
Quantity
1 vial (1 mL)
50,000 U
Items
Endoglycosidase H (Endo H) (500 U/μL)
Quantity
1 vial (50,000 U)
Items
10× Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
Items
10× Reaction buffer
Quantity
1 vial (1 mL)
