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Leadgene Leadgene
Endoglycosidase H (Endo H)
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Catalog Number

LDG0002RG

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  • Overview

    Description

    Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.

    Product Note

    • Please fine-tune the input sample volume to find the optimal condition for your assay.
    • Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein.

    Components

    Package

    Items

    Quantity

    10,000 U

    Endoglycosidase H (Endo H) (500 U/μL)

    1 vial (10,000 U)

    10× Glycoprotein Denaturing Buffer

    1 vial (1 mL)

    10× Reaction buffer

    1 vial (1 mL)

    50,000 U

    Endoglycosidase H (Endo H) (500 U/μL)

    1 vial (50,000 U)

    10× Glycoprotein Denaturing Buffer

    1 vial (1 mL)

    10× Reaction buffer

    1 vial (1 mL)

    • 10,000 U

      Items

      Endoglycosidase H (Endo H) (500 U/μL)

      Quantity

      1 vial (10,000 U)

      Items

      10× Glycoprotein Denaturing Buffer

      Quantity

      1 vial (1 mL)

      Items

      10× Reaction buffer

      Quantity

      1 vial (1 mL)

    • 50,000 U

      Items

      Endoglycosidase H (Endo H) (500 U/μL)

      Quantity

      1 vial (50,000 U)

      Items

      10× Glycoprotein Denaturing Buffer

      Quantity

      1 vial (1 mL)

      Items

      10× Reaction buffer

      Quantity

      1 vial (1 mL)

  • Specifications
    • Expression System

      Escherichia coli

      Concentration

      500 U/μL

    • Storage Buffer

      Endoglycosidase H is supplied in 20 mM Tris-HCl, 50 mM NaCl and 5 mM EDTA, pH 7.5

      Purity

      >95% as determined by SDS-PAGE analysis.

    • Unit Definition

      One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.

      Endotoxin Level

      <1 EU per 1 μg of the protein by the LAL method.

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C for -80°C long-term storage under sterile conditions.

      Avoid repeated free-thaw cycles.

  • Image
    The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

    The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

    SDS-PAGE analysis of recombinant Endoglycosidase H

    SDS-PAGE analysis of recombinant Endoglycosidase H

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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