Description
Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.
Leadgene® Endoglycosidase H (Endo H) ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Endo H level in sample solution. The Endoglycosidase H ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Test Principle
Endoglycosidase H ELISA kit is used to detect Endo H in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-Endo H monoclonal antibody to the solid phase. Endo H in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-Endo H monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of Endo H level is accomplished by comparing the absorbance with standard curve.
