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Leadgene Leadgene
C. difficile Toxin B Protein, His tag, E. coli
Catalog Number

LDG024POE

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  • Specifications
    • Species of Origin

      Clostridium difficile

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 63.93 kDa.
      The protein migrates as 60 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Clostridium difficile enterotoxin B is a protein produced by the bacterium Clostridium difficile. It plays a crucial role in the pathogenesis of C. difficile-associated diseases, particularly in causing damage to the intestinal mucosa and inducing inflammation. This toxin disrupts cellular integrity by targeting specific receptors on the host cell surface, leading to cell death and tissue damage. Understanding the mechanisms of action of this toxin is essential for developing effective treatments and vaccines against C. difficile infections.

      Synonyms

      Glucosyltransferase TcdB, toxB

    • Uniprot ID

      P18177

      Sequence Note

      Met1-Leu543

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant toxin B.

    SDS-PAGE analysis of recombinant toxin B.

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  • Datasheet & Documents
    MSDS
    Manual
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