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Leadgene Leadgene
Anti-ZIKV NS1 Antibody [Clone ZK243]
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Catalog Number

LDG0016YA

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  • Overview

    Description

    Zika virus belongs to the family Flaviviridae, genus Flavivirus. Zika virus is transmitted primarily by mosquitos, but also can be transmitted through sexual, vertical, and blood transfusion. NS1 is a highly-conserved viral protein in flaviviruses. In infected patients, the concentration of NS1 protein in circulating blood is relatively high, and can be detected up to 5 days after the onset of illness.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2a

      Clone Name

      clone ZK243

    • Immunogen

      Zika virus NS1

      Reactivity

      Zika virus

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      NS1

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    ELISA titration of Anti-ZIKV NS1 Antibody [Clone ZK243] Titration curve of anti-ZIKV NS1 antibody in ELISA. Red: ZIKV NS1; Black: BSA (negative control).

    ELISA titration of Anti-ZIKV NS1 Antibody [Clone ZK243]
    Titration curve of anti-ZIKV NS1 antibody in ELISA. Red: ZIKV NS1; Black: BSA (negative control).

    Immunofluorescence analysis of Anti-ZIKV NS1 Antibody [Clone ZK243] ZIKV NS1 mAb (1:400) were used for detecting ZIKV NS1 in IFA. Scale bar: 200 μm

    Immunofluorescence analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
    ZIKV NS1 mAb (1:400) were used for detecting ZIKV NS1 in IFA. Scale bar: 200 μm

    FACS analysis of Anti-ZIKV NS1 Antibody [Clone ZK243] ZIKV or DENV-infected Vero cells were stained with mouse anti-ZIKV NS1 monoclonal antibody at 5 μg/mL (red) and without antibody control (black).

    FACS analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
    ZIKV or DENV-infected Vero cells were stained with mouse anti-ZIKV NS1 monoclonal antibody at 5 μg/mL (red) and without antibody control (black).

    ELISA assay of Anti-ZIKV NS1 Antibody [Clone ZK243] Indirect ELISA analysis was performed by coating a plate with recombinant NS1 protein of ZIKV, CHIKV or DENV and probing with anti-ZIKV NS1 Antibody.

    ELISA assay of Anti-ZIKV NS1 Antibody [Clone ZK243]
    Indirect ELISA analysis was performed by coating a plate with recombinant NS1 protein of ZIKV, CHIKV or DENV and probing with anti-ZIKV NS1 Antibody.

    Western blotting analysis of Anti-ZIKV NS1 Antibody [Clone ZK243] Western blot: Virus infection Vero cell lysates were stained with mouse anti-ZIKV NS1 monoclonal antibody at 1:2000 dilution. Lane 1: Vero mock cell lysate (4 μg), Lane 2: Vero ZIKV cell lysate (4 μg), Lane 3: Vero DENV1 cell lysate (4 μg), Lane 4: Vero DENV2 cell lysate (4 μg), Lane 5: Vero DENV3 cell lysate (4 μg), Lane 6: Vero DENV 4 cell lysate (4 μg).

    Western blotting analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
    Western blot: Virus infection Vero cell lysates were stained with mouse anti-ZIKV NS1 monoclonal antibody at 1:2000 dilution. Lane 1: Vero mock cell lysate (4 μg), Lane 2: Vero ZIKV cell lysate (4 μg), Lane 3: Vero DENV1 cell lysate (4 μg), Lane 4: Vero DENV2 cell lysate (4 μg), Lane 5: Vero DENV3 cell lysate (4 μg), Lane 6: Vero DENV 4 cell lysate (4 μg).

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  • Datasheet & Documents
    MSDS
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