Anti-SARS-CoV-2 Spike Antibody [Clone LGSV201]

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Catalog Number

LDG0086YA

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• Overview

  Description

  Human anti-SARS-CoV-2 Spike Antibody [CR3022] recognizes human SARS-CoV and CoV-2 Spike protein with high affinity. The binding site is amino acids 318-510 (RBD, Receptor Binding Domain) in the S1 subunit of the spike protein. Coronavirus spike protein conducts the process that interacts with cellular receptor and membrane fusion to allow virus entering into target cells. Spike protein also can be used to define the specificity of the virus and be used as a critical target for vaccine design. The glycosylated spike protein can be detected in the virus-infected cell and cell culture medium. The RBD is responsible for recognizing the cell surface receptor.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB:1: 1000-5000
  IFA:1: 500-1000
  FACS: Assay dependent
  
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG1

    Clone Name

    clone LGSV201

  • Immunogen

    SARS-CoV-2 VLP

    Reactivity

    SARS-CoV & CoV-2

  • Application

    ELISA, WB, IFA

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Storage Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    Spike protein

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000)
  Lane: SARS-CoV-2 VLP

  LDG0086YAWB01 Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000)Lane 1: SARS-CoV-2 S1-RBDLane 2: SARS-CoV-2 S1Lane 3: SARS-CoV S1-RBDLane 4: SARS-CoV S1Lane 5: MERS-CoV S1-RBD

  Immunofluorescence analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:1000)

  ELISA titration of Mouse anti-SARS-CoV-2 Spike mAb

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.