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Leadgene Leadgene
Anti-PGA5 Antibody [Clone 85-5]
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Catalog Number

LDG0054YA

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  • Overview

    Description

    Pepsinogens are members of the peptidases that are secreted by gastric chief cells and are inactive polypeptide proenzymes known as zymogens. They are converted by gastric acid to active pepsins, which are involved in the digestion of proteins. Pepsinogen A5 (PGA5), an isozymogen of pepsinogen A, is the predominant endopeptidase in the gastric acid and is associated with atrophic gastritis and gastritis.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:100-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 85-5

    • Immunogen

      The immunogen information used to generate this antibody is proprietary.

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      PGA5

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-PGA5 Antibody [Clone 85-5] HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-PGA5 monoclonal antibody (1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-PGA5 Antibody [Clone 85-5]
    HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
    Triton X-100. Cells were stained with mouse anti-PGA5 monoclonal antibody
    (1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
    1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    FACS analysis of Anti-PGA5 Antibody [Clone 85-5] K562 cells were stained with mouse anti-PGA5 monoclonal antibody at 2 μg/ml (red) and without antibody control (black).

    FACS analysis of Anti-PGA5 Antibody [Clone 85-5]
    K562 cells were stained with mouse anti-PGA5 monoclonal antibody at 2
    μg/ml (red) and without antibody control (black).

    ELISA analysis of Anti-PGA5 Antibody [Clone 85-5] The specificity of anti-PGA5 antibody recognize recombinant proteins in ELISA. Red: Porcine pepsin; Orange: PGA5; Green: Pepsinogen I; Blue: Pepsinogen II; Black: BSA (negative control).

    ELISA analysis of Anti-PGA5 Antibody [Clone 85-5]
    The specificity of anti-PGA5 antibody recognize recombinant proteins in
    ELISA. Red: Porcine pepsin; Orange: PGA5; Green: Pepsinogen I; Blue:
    Pepsinogen II; Black: BSA (negative control).

    Western blotting analysis of Anti-PGA5 Antibody [Clone 85-5] Recombinant proteins and mouse stomach lysates were stained with mouse anti-PGA5 monoclonal antibody at 1:5000 dilution. Lane 1: Purified human pepsinogen I (25 ng). Lane 2: Purified human pepsinogen II (25 ng). Lane 3: Mouse stomach lysate (30 μg). Lane 4: Human gastric juice (10 μL).

    Western blotting analysis of Anti-PGA5 Antibody [Clone 85-5]
    Recombinant proteins and mouse stomach lysates were stained with mouse
    anti-PGA5 monoclonal antibody at 1:5000 dilution. Lane 1: Purified human
    pepsinogen I (25 ng). Lane 2: Purified human pepsinogen II (25 ng). Lane 3:
    Mouse stomach lysate (30 μg). Lane 4: Human gastric juice (10 μL).

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  • Datasheet & Documents
    MSDS
    Manual
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