Leadgene Leadgene
Anti-mCherry Antibody Sepharose Purification Kit
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  • Overview


    Anti-mCherry sepharose, is consist of an mCherry VHH coupled to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or mammalian expression proteins. The mCherry epitope system relies on a VHH mCherry recombinant antibody, which is able to react with N- and C- terminal RFP, DsRed and mCherry tagged fusion proteins. This product can be used for the immuneprecipitation or immune affinity purification.
    The purified antibody is immobilized at 2-4 mg antibody per ml of 50% slurry and this kit allows a rapid and efficient affinity purification of active mCherry fusion proteins. The affinity resin allows an efficient binding of mCherry fusion proteins without the need for preliminary steps and calibrations. The affinity bound mCherry fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.

    Product Note

    The optimal protein concentration of the cell lysate should be determined by the end user.

  • Specifications
    • Clonality



      Immunoprecipitation, Immunoaffinity Purification

    • Conjugation



      Recognizes RFP, DsRed and mCherry tag on either the N- or C-terminus of the recombinant fusion protein.

    • Sample Type

      Cell lysate

      Binding capacity

      The binding and elution capacity of 1 mL settled Anti-mCherry sepharose may be vary, commonly more than 1 mg RFP, DsRed or mCherry fusion proteins.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product

      Please refer to product manual for storage constructions

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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