Anti-IL-8 Antibody [Clone 58-1]

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Catalog Number

LDG0030YA

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• Overview

  Description

  The CXC chemokine is a group of secreted proteins, attracts neutrophils and lymphocytes. Interleukin-8 (IL-8, or CXCL8), a member of the CXC chemokines, is a proinflammatory cytokine produced by macrophages and other cell types like endothelial cells, epithelial cells and airways smooth muscle cells. This cytokine mainly attracts and activates neutrophils to the site of inflammation and also plays an important role in cell proliferation, tissue remodeling and angiogenesis.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:500-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG1

    Clone Name

    clone 58-1

  • Immunogen

    Interleukin 8

    Reactivity

    Human

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Storage Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    Interleukin 8

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescence analysis of Anti-IL-8 Antibody [Clone 58-1]
  HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-IL-8 monoclonal antibody
  (1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-IL-8 Antibody [Clone 58-1]
  Human peripheral blood mononuclear cells (PBMC) were stained with mouse
  anti-IL-8 monoclonal antibody at 2 μg/ml (red) and without antibody control
  (black).

  ELISA titration of Anti-IL-8 Antibody [Clone 58-1]
  Titration curve of anti-IL-8 antibody in ELISA. Red: IL-8; Black: BSA (negative
  control).

  Western blotting analysis of Anti-IL-8 Antibody [Clone 58-1]
  Recombinant IL-8 protein (100 ng) was stained with mouse anti-IL-8
  monoclonal antibody at 1:5000 dilution.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.