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TW
Leadgene Leadgene
Anti-IL-6 Antibody [Clone 3-5]
Catalog Number

LDG0180YA

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  • Overview

    Description

    Interleukin-6 (IL-6) is a pleiotropic, 22-28 kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.

    Product Note

    Recognize IL-6 in ELISA, when clone 3-5 antibody was paired with Anti-IL-6 Antibody [clone 9D9] (cat. LDG0026YA).

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-1:5000
    IFA: 1:500-1:1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 3-5

    • Immunogen

      Recombinant human IL-6 protein

      Reactivity

      Human

    • Application

      ELISA

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      Interleukin 6

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    ELISA titration of Anti-IL-6 Antibody [Clone 3-5] Titration curve of anti-IL-6 antibody in ELISA. Red: IL-6; Green: BSA (negative control).

    ELISA titration of Anti-IL-6 Antibody [Clone 3-5]
    Titration curve of anti-IL-6 antibody in ELISA. Red: IL-6; Green: BSA (negative control).

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  • Datasheet & Documents
    MSDS
    Manual
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