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Catalog Number

LDG0033YA

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Overview

Description

Interleukin-33 (IL-33), a member of the IL-1 family, is a nuclear alarmin cytokine expressed by various cell types. Unlike most other cytokines, IL-33 lacks a secretory signal peptide, and thus it is passively released during cell damage or tissue injury. IL-33 plays a crucial role in type-2 innate immunity through interaction with the ST2 receptor.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:400-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG2a
  
  Clone Name
  
  clone 7E8
- Immunogen
  
  Interleukin 33
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Storage Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  Interleukin 33
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-IL-33 Antibody [Clone 7E8]
HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-IL-33 monoclonal antibody
(1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

FACS analysis of Anti-IL-33 Antibody [Clone 7E8]
HeLa cells were stained with mouse anti-IL-33 monoclonal antibody at 1
μg/ml (red) and without antibody control (black).

ELISA titration of Anti-IL-33 Antibody [Clone 7E8]
Titration curve of anti-IL-33 antibody in ELISA. Red: IL-33; Black: BSA
(negative control).

Western blotting analysis of Anti-IL-33 Antibody [Clone 7E8]
Recombinant IL-33 protein (50 ng) was stained with mouse anti-IL-33
monoclonal antibody at 1:5000 dilution.

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Datasheet & Documents

Datasheet

MSDS

Manual

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