Anti-DENV NS5 Antibody [Clone 37-2]

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Catalog Number

LDG0007YA

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• Overview

  Description

  Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. NS5 is the largest and the most conserved protein of Dengue virus, acting as both a RNA-dependent RNA polymerase and a RNA methyltransferase enzyme. The NS5 proteins are important for viral replication and can down-regulate the host immune interferon response.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:500-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2b

    Clone Name

    clone 37-2

  • Immunogen

    Dengue virus NS5

    Reactivity

    Dengue virus

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Storage Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    NS5

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescence analysis of Anti-DENV NS5 Antibody [Clone 37-2]
  Control Vero cells (uninfected) and Virus (DENV 1,2,4) infection Vero cells
  were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-
  100. Cells were stained with mouse anti-DENV NS5 monoclonal antibody
  followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400,
  green) and cell nuclei were stained with Hoechst 33342 (Blue).

  ELISA titration of Anti-DENV NS5 Antibody [Clone 37-2]
  Titration curve of anti-DENV NS5 antibody in ELISA. Red: NS5; Black: BSA
  (negative control).

  Western blotting analysis of Anti-DENV NS5 Antibody [Clone 37-2]
  Virus infection Vero cell lysates were stained with mouse anti-DENV NS5
  monoclonal antibody at 1:1000 dilution. Lane 1: Vero cell lysate (10 μg), Lane
  2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2
  infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection
  (10 μg).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.