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Species of Origin
Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2)
Expression System
Escherichia coli
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Affinity Tag
His Tag (N-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>90% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 82.8 kDa.
The protein migrates as 75 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin Level
<1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
Adeno-associated virus 2 Capsid protein VP1 is a vital component of the AAV2 viral capsid. It facilitates viral entry into host cells by interacting with cell surface receptors and aids in packaging the viral genome. VP1's structure and interactions with host factors influence viral infectivity and determine the efficacy of AAV2-based gene therapy approaches. Understanding VP1's role is crucial for optimizing vector design and enhancing therapeutic outcomes in gene therapy applications.
Synonyms
Capsid protein VP1
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Uniprot ID
P03135
Sequence Note
Met1-Leu735
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.