Description
The HyLink™ BirA Biotin Labeling Kit for Avi-tag Proteins provides a highly efficient, specific, and reproducible solution for biotinylating recombinant proteins or peptides containing Avi-tags or other enzymatic biotinylation tags such as Biotag™. This kit utilizes the biotin ligase enzyme BirA, which specifically and uniformly attaches biotin to a lysine residue within the Avi-tag sequence. Compared to traditional chemical biotinylation methods, this enzymatic approach offers site-specific labeling, minimizes variability between batches, and preserves the biological activity and structural integrity of the target protein.
Components
GST-BirA (lyophilized)
Amount
40 μg
Quantity
1 vial
Reconstitution buffer
Amount
0.2 mL
Quantity
1 vial
10X Reaction buffer
Amount
1.5 mL
Quantity
1 vial
SpinDesalt Column
Amount
pcs
Quantity
16 pcs
