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Species of Origin
Human
Expression System
CHO
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
The purity of Human CD16A Protein is greater than 90% as determined by SEC-HPLC.
Molecular weight
The protein has a calculated 22.66 MW of kDa.
The protein migrates as 30-45 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to bind human IgG in functional ELISA. Immobilized human IgG at 0.5 μg/mL (100μL/well) can bind Recombinant Human CD16A Protein.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Mycoplasma
Not detected
Form
Lyophilized

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Specifications
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Background
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Background
CD16A, also known as FcγRIIIa, is an Fc receptor involved in antibody-dependent cellular cytotoxicity (ADCC). It is critical in immune responses, particularly in cancer and infectious disease therapies.
Synonyms
Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a
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Uniprot ID
P08637
Sequence Note
Gly17-Gln208
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Review
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.