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Leadgene Leadgene
Human CD16A, His Tag, CHO
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Catalog Number

LDG114PHM

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  • Specifications
    • Species of Origin

      Human

      Expression System

      CHO

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      The purity of Human CD16A Protein is greater than 90% as determined by SEC-HPLC.

      Molecular weight

      The protein has a calculated 22.66 MW of kDa.
      The protein migrates as 30-45 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to bind human IgG in functional ELISA. Immobilized human IgG at 0.5 μg/mL (100μL/well) can bind Recombinant Human CD16A Protein.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Lyophilized

  • Background
    • Background

      CD16A, also known as FcγRIIIa, is an Fc receptor involved in antibody-dependent cellular cytotoxicity (ADCC). It is critical in immune responses, particularly in cancer and infectious disease therapies.

      Synonyms

      Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a

    • Uniprot ID

      P08637

      Sequence Note

      Gly17-Gln208

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    HPLC analysis of recombinant Human CD16A, His Tag, CHO. The purity of Human CD16A Protein is greater than 90% as determined by SEC-HPLC.

    HPLC analysis of recombinant Human CD16A, His Tag, CHO.
    The purity of Human CD16A Protein is greater than 90% as determined by SEC-HPLC.

    SDS-PAGE analysis of recombinant human CD16A.

    SDS-PAGE analysis of recombinant human CD16A.

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  • Datasheet & Documents
    MSDS
    Manual
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