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Leadgene Leadgene
LeadGMP® Human IL-4 Protein, His Tag, E. coli
Catalog Number

LDG005PHE-GMP

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 15.9 kDa.
      The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is < 0.2 ng/mL. The specific activity of recombinant human IL-4 is approximately > 1 x 10⁷ IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656).

      Endotoxin level

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Lyophilized

  • Background
    • Background

      Interleukin-4 (IL-4) is a key cytokine produced by activated T cells, mast cells, basophils, neutrophils and eosinophils. IL-4 is critical for the development of Th2-mediated responses, which is related to allergy and asthma. It can also regulate B cell responses, including survival, cell proliferation and gene expression. IL-4 also plays fundamental role for B-cell stimulation, including induction of the IgE isotype switch.

      Synonyms

      Interleukin-4, B-cell stimulatory factor 1 , BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra

    • Uniprot ID

      P05112

      Sequence Note

      His25-Ser153

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

      Manufacturing specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    SDS-PAGE analysis of LeadGMP® IL-4, Human

    SDS-PAGE analysis of LeadGMP® IL-4, Human

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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