Protein A is a membrane protein found in the cell wall of Staphylococcus aureus. Protein A contains four binding sites with a high affinity to human IgG, rabbit IgG and others and is therefore suitable for immunoprecipitation assay and purification of immunoglobulins.
Protein G is a surface protein found in the cell wall of Streptococcal bacteria. Protein G has a high affinity to human IgG, mouse IgG, and others and is, therefore, suitable for purifying and detecting immunoglobulins.
Protein A/G has broader binding ability than either Protein A or G alone. Protein A/G is a great tool for the purification and detection of all humans, rabbit, and mouse IgG subclasses, IgA, IgE, and IgM without interference from these other serum proteins.
Addition: MOPS SDS Running Buffer, 2 Packs (for 2 L buffer preparation).
Add 1 L of double-distilled water to 1 pack of MOPS SDS Running Buffer and homogenize by using a vortex mixer, which is used as working running buffer.
Once prepared, the buffer should be stored at 2-8°C and must be used within one week.