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Leadgene Leadgene
Human Sonic Hedgehog (C24II), Tag Free, HEK293
Catalog Number

LDG017PVM

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  • Specifications
    • Species of Origin

      Human

      Expression system

      HEK293

    • Affinity Tag

      Tag Free

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.67 kDa.
      The protein migrates as 15-25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.5-2 µg/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Sonic hedgehog protein (SHH) plays a critical role in development of embryonic morphogenesis which mediate the process of organogenesis and central nervous system. The sonic hedgehog signal pathway also plays an important role in cancerous tumors like embryonic cerebellar tumor, prostate cancer tumours and medulloblastoma. Shh binds the receptor (Ptc1) that induce the smo protein to activate the downstream pathway, which induce the shh target gene expression.

      Synonyms

      Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC

    • Uniprot ID

      #Q15465

      Sequence Note

      Gly25-Gly197

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of Human Sonic Hedgehog (C24II).

    SDS-PAGE analysis of Human Sonic Hedgehog (C24II).

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  • Datasheet & Documents
    MSDS
    Manual
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