Species of Origin
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
>95% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 19.67 kDa.
The protein migrates as 15-25 kDa under reducing condition (SDS-PAGE analysis).
Measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.5-2 µg/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Sonic hedgehog protein (SHH) plays a critical role in development of embryonic morphogenesis which mediate the process of organogenesis and central nervous system. The sonic hedgehog signal pathway also plays an important role in cancerous tumors like embryonic cerebellar tumor, prostate cancer tumours and medulloblastoma. Shh binds the receptor (Ptc1) that induce the smo protein to activate the downstream pathway, which induce the shh target gene expression.
Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
SDS-PAGE analysis of Human Sonic Hedgehog (C24II).1/1
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.