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Species of Origin
Human
Expression system
CHO
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 16.76 kDa.
The protein migrates as 30-40 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to bind human PD-L1 in functional ELISA. Immobilized Recombinant human PD-L1 Protein at 0.5 μg/mL (100μL/well) can bind Recombinant Human PD-1 Protein.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
PD-1, or programmed cell death protein 1, is a cell surface receptor protein primarily expressed on activated T cells. It plays a pivotal role in regulating immune responses by interacting with its ligands, PD-L1 and PD-L2, which are often expressed on cancer cells and antigen-presenting cells. Binding of PD-1 to its ligands leads to inhibition of T cell activation and proliferation, suppressing immune responses against cancer cells and contributing to immune evasion. Blocking the PD-1/PD-L1 interaction with therapeutic antibodies enhances T cell activity, making PD-1 inhibitors a promising approach in cancer immunotherapy.
Synonyms
Programmed cell death protein 1, CD279
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Uniprot ID
NP_005009.2
Sequence Note
Met1-Gln167
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![HPLC analysis of recombinant Human PD-1, His Tag, CHO. The purity of Human PD-1 Protein is greater than 95% as determined by RP-HPLC.]()
HPLC analysis of recombinant Human PD-1, His Tag, CHO.
The purity of Human PD-1 Protein is greater than 95% as determined by RP-HPLC.![SDS-PAGE analysis of recombinant Human PD-1.]()
SDS-PAGE analysis of recombinant Human PD-1.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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