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Leadgene Leadgene
Human LIGHT, His Tag, E. coli
Catalog Number

LDG125PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 20.30 kDa.
      The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce cytotoxicity in HT-29 cells in the presence of IFN-gamma. The ED₅₀ for this effect is < 10 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      LIGHT, also known as TNFSF14 is a member of the TNF superfamily that produced by multiple immune cells such as activated T cells and immature dendritic cells (DCs). LIGHT is a 29 kDa type II transmembrane protein, which serves as a ligand for both lymphotoxin β receptor (LTβR) and TNFSF signaling receptors (TNFRSF14/HVEM). Besides, LIGHT can amplify NF-kB signaling pathway in T cells when presenting anti-CD3 antibody treatment. Additionally, LIGHT can stimulate T cell proliferation and IFN-expression via interacting with TNFRS13 /HVEM.

      Synonyms

      LIGHT, Herpes virus entry mediator ligand, HVEM-L, CD258, Tumor necrosis factor ligand superfamily member 14

    • Uniprot ID

      #O43557

      Sequence Note

      Arg64-Val240

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human LIGHT.

    SDS-PAGE analysis of recombinant human LIGHT.

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  • Datasheet & Documents
    MSDS
    Manual
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