Leadgene Leadgene
Human IL-29, His Tag, E. coli
Catalog Number

LDG033PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 20.70 kDa.
      The protein migrates as 22 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce IL-8 secretion in HuH7 cells. The ED₅₀ for this effect is <6 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 29 (IL-29) is a cytokine, predicts a molecular mass of 21.9 kDa. It belongs to type III interferons group, also termed interferons λ (IFN-λ). Its induction of STAT3-STAT5 has also been displayed, albeit to a lesser degree. The STAT1 /STAT2 signaling cascade transpires as follows: once tyrosine residues on STAT1 and STAT2 are phosphorylated, these proteins dimerize and are subsequently transported to the nucleus.

      Synonyms

      IFN-lambda-1, Cytokine Zcyto21, Interleukin-29, IL-29

    • Uniprot ID

      Q8IU54

      Sequence Note

      Pro23-Thr200

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human IL-29.

    SDS-PAGE analysis of recombinant human IL-29.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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