Leadgene Leadgene
Human IL-27 (EBI3), His Tag, E. coli
Catalog Number

LDG031PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 24.25 kDa.
      The protein migrates as 25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce TF-1 cells proliferation using a concentration range of 20-200 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 27 EBI3 (IL-27 EI3) predicts a molecular mass of 23.4 kDa. IL-27 is a heterodimeric cytokine that is encoded by Epstein-Barr virus-induced gene 3 (EBI3) and IL-27p28. It is expressed by antigen presenting cells and interacts with a specific cell-surface receptor complex known as IL-27 receptor (IL-27R).

      Synonyms

      IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein

    • Uniprot ID

      Q14213

      Sequence Note

      Arg21-Lys229

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human IL-27 (EBI3).

    SDS-PAGE analysis of recombinant human IL-27 (EBI3).

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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