Human IGF-II, His Tag, E. coli

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Catalog Number

LDG089PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 8.28 kDa.
    The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is <3 ng/mL. The specific activity of recombinant human IGF-II is > 3x 10⁵ IU/mg.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Insulin like Growth Factors 2 (IGF-II) is a 7.48 kDa member of the Insulin-like Growth Factors with 67 amino acid residues. IGF-II is mainly expressed from placenta, extravillous trophoblasts, leydig cells, syncytiotrophoblasts, cytotrophoblasts, peritubular cells. IGF-II regulating fetoplacental development and tissue differentiation. In adults, IGF-II signaling involves glucose metabolism in adipose tissue, skeletal muscle and liver. It also has important implications for metabolic disorders and cancer.

    Synonyms

    Insulin-like Growth Factors II, Somatomedin-A, T3M-11-derived Growth Factors, Insulin-like Growth Factors II Ala-25 Del, Preptin

  • Uniprot ID

    #P01344

    Sequence Note

    Ala25-Glu91

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human IGF-II, His Tag, E. coli (LDG089PHE) induced MCF-7 cell proliferation, with the ED50 at 2.349 ng/mL.

  SDS-PAGE analysis of recombinant human IGF-II.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.