Human Fibronectin, His Tag, E. coli

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Catalog Number

LDG193PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 64 kDa.
    The protein migrates as 60-65 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is <100 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Mycoplasma

    Not detected

    Form

    Lyophilized

• Background

  • Background

    Fibronectin is a high-molecular-weight extracellular matrix glycoprotein involved in various cellular processes including adhesion, migration, growth, and differentiation. It plays crucial roles in wound healing, tissue repair, angiogenesis, and is implicated in cancer progression and fibrosis.

    Synonyms

    FN, FN1, CIG, FINC, FNZ, MSF, GFND, GFND2, LETS

  • Uniprot ID

    P02751-15

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is <100 ng/mL.
  Activity analysis of recombinant Human Fibronectin, His Tag, E. coli.

  SDS-PAGE analysis of recombinant human fibronectin protein.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.