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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 8.55 kDa.
The protein migrates as 10 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to chemoattract BaF3 cells transfected with human CXCR4. The ED₅₀ for this effect is <0.5 ng/mL.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
C-X-C motif chemokine 12 (CXCL12) also named stromal cell-derived factor 1 (SDF-1), which is a chemokine of the intercrine alpha family. CXCL12 is a 19.5kDa protein containing 167 amino acid residues. The function of CXCL12 include the hematopoiesis, angiogenesis embryogenesis, inflammation and angiogenesis. CXCL12 activated by the IL-1, TNF or lipopolysaccharide and induce the immune response in leukocytes.
Synonyms
Stromal cell-derived factor 1, SDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, SDF-1-beta, 3-72, SDF-1-alpha, 3-67
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Uniprot ID
#P48061
Sequence Note
Val24-Asn88
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Review
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.