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Leadgene Leadgene
Human CXCL12 (aa 24-88), His Tag, E. coli
Catalog Number

LDG053PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 8.55 kDa.
      The protein migrates as 10 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to chemoattract BaF3 cells transfected with human CXCR4. The ED₅₀ for this effect is <0.5 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      C-X-C motif chemokine 12 (CXCL12) also named stromal cell-derived factor 1 (SDF-1), which is a chemokine of the intercrine alpha family. CXCL12 is a 19.5kDa protein containing 167 amino acid residues. The function of CXCL12 include the hematopoiesis, angiogenesis embryogenesis, inflammation and angiogenesis. CXCL12 activated by the IL-1, TNF or lipopolysaccharide and induce the immune response in leukocytes.

      Synonyms

      Stromal cell-derived factor 1, SDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, SDF-1-beta, 3-72, SDF-1-alpha, 3-67

    • Uniprot ID

      #P48061

      Sequence Note

      Val24-Asn88

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human CXCL12 (aa 24-88).

    SDS-PAGE analysis of recombinant human CXCL12 (aa 24-88).

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  • Datasheet & Documents
    MSDS
    Manual
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