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Leadgene Leadgene
Human CD326, His Tag, E. coli
Catalog Number

LDG066PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 28.36 kDa.
      The protein migrates as 30 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by the ability of the immobilized protein to support the adhesion of the 3T3 cells. The ED₅₀ for this effect is 0.2-1.7 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Human CD326 also called epithelial cellular adhesion molecule (EpCAM), which is a 28 kDa protein containing 242 amino acid residues. Human CD326 is a transmembrane glycoprotein, which is a marker of cancer. CD326 induces the expression of c-myc, cyclins A & E and e-fabp which is involved the cancer cell proliferation and migration

      Synonyms

      Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial glycoprotein , EGP, Epithelial glycoprotein 314 , EGP314,  hEGP314, KS 1 /4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Epithelial cell adhesion molecule, Ep-CAM

    • Uniprot ID

      #P16422

      Sequence Note

      Gln24-Lys265

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human CD326.

    SDS-PAGE analysis of recombinant human CD326.

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  • Datasheet & Documents
    MSDS
    Manual
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