Language
EN
Leadgene Leadgene
Human Activin B, His Tag, E. coli
More Images
Catalog Number

LDG064PHE

More Documents
Loading package & price...
  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 13.75 kDa.
      The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <0.7 ng/mL. The specific activity of recombinant human Activin B is > 1.5 x 10⁶ IU/mg.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Activin B is the cytokine of the TGF-beta family, which is a 13 kDa protein containing 115 amino acid residues. Activin B has the wide variety of biological function including stem cell differentiation, inflammation, bone remodeling and neural development. They also have a role in regulation of FSH secretion. Activin B induces the phosphorylation of Smad which binds the smad binding element.

      Synonyms

      Inhibin beta B chain, Activin beta-B chain, INHBB

    • Uniprot ID

      #P09529

      Sequence Note

      Gly293-Ala407

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human Activin B, His Tag, E. coli (LDG064PHE) induced hemoglobin expression in K562 cells, with the ED50 at 0.651 ng/mL.

    Human Activin B, His Tag, E. coli (LDG064PHE) induced hemoglobin expression in K562 cells, with the ED50 at 0.651 ng/mL.

    SDS-PAGE analysis of recombinant human Activin B.

    SDS-PAGE analysis of recombinant human Activin B.

    1
    /
    2
    Total   (2)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.