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Leadgene Leadgene
HCoV-NL63 Nucleocapsid Protein, His-SUMO Tag, HEK293
Catalog Number

LDG003PVM

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  • Specifications
    • Species of Origin

      Human coronavirus NL63

      Expression system

      HEK293 cell

    • Affinity Tag

      His-SUMO Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 53.79 kDa.
      The protein migrates about 70-75 kDa under reducing condition (SDS-PAGE analysis).

    • Form

      Lyophilized

  • Background
    • Background

      There are seven human coronaviruses have been identified. The common human coronaviruses are four groups, known as 229E (α coronavirus), NL63 (α coronavirus), OC43 (β coronavirus) and HKU1 (β coronavirus). Because the crown-like spikes on the surface of virus, they are named for coronaviruses. HCoVs cause the respiratory tract diseases, especially severe in infants and the elderly. The spike protein controled the infection of target cells and it facilitated entry into cells by binding cellular receptors.

      Synonyms

      Nucleoprotein, N, Nucleocapsid protein, NC Protein N

    • Uniprot ID

      #Q6Q1R8

      Sequence Note

      Met1-His377

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of Human Coronavirus (NL63) nucleocapsid protein

    SDS-PAGE analysis of Human Coronavirus (NL63) nucleocapsid protein

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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