Exonuclease V (RecBCD)

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Catalog Number

LDG0041RG

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• Overview

  Description

  Exonuclease V (RecBCD) is a highly processive, ATP-dependent recombinant enzyme complex from Escherichia coli that possesses dual helicase and exonuclease activities. Driven by ATP hydrolysis, it rapidly degrades linear single- and double-stranded DNA from both 5' and 3' termini into short, acid-soluble oligonucleotides. With strict specificity for free ends, the enzyme degrades linear DNA while generally preserving nicked and supercoiled plasmid DNA. This unique substrate discrimination makes Exonuclease V an essential tool for eliminating contaminating linear genomic DNA to yield high-purity circular DNA for sensitive downstream applications.

  Components

  Item	Quantity
  Exonuclease V (RecBCD) (20 U/μL)	1 vial (250 μL)
  10X Reaction buffer	1 vial (1 mL)
  10 mM ATP	1 vial (1 mL)

  • Exonuclease V (RecBCD) (20 U/μL)

    Quantity

    1 vial (250 μL)

  • 10X Reaction buffer

    Quantity

    1 vial (1 mL)

  • 10 mM ATP

    Quantity

    1 vial (1 mL)

• Specifications

  • Species of Origin

    Escherichia coli

    Expression system

    Escherichia coli

  • Concentration

    20 U/μL

    Buffer

    Exonuclease V is supplied in 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol, pH 7.5.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit of Exonuclease V digests 0.33 μg of linear double-stranded DNA into acid-soluble nucleotides in 30 minutes at 37°C under standard assay conditions.

  • Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Liquid

• Background

  • Synonyms

    Exonuclease V, Exodeoxyribonuclease V, RecBCD, RecBCD enzyme, RecBCD complex, RecBCD nuclease, RecBCD helicase-nuclease, Helicase/nuclease RecBCD, ExoV, ATP-dependent exonuclease V, ATP-dependent DNA exonuclease, Exodeoxyribonuclease V complex, RecB-RecC-RecD complex, DNA repair helicase-nuclease, EC 3.1.11.5

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable at -20°C for 12 months under sterile conditions.

• Image

  Agarose gel electrophoresis of DNA digested with Exonuclease V.
  The standard assay was performed by incubating 1 unit of Exonuclease V and 0.33 μg of linear dsDNA at 37°C for 30 minutes.

  SDS-PAGE analysis of recombinant Exonuclease V (RecBCD).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.

最後更新：2026.10.15