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LDG0030YA

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Overview

Description

The CXC chemokine is a group of secreted proteins, attracts neutrophils and lymphocytes. Interleukin-8 (IL-8, or CXCL8), a member of the CXC chemokines, is a proinflammatory cytokine produced by macrophages and other cell types like endothelial cells, epithelial cells and airways smooth muscle cells. This cytokine mainly attracts and activates neutrophils to the site of inflammation and also plays an important role in cell proliferation, tissue remodeling and angiogenesis.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG1
  
  Clone Name
  
  clone 58-1
- Immunogen
  
  Interleukin 8
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  Interleukin 8
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-IL-8 Antibody [Clone 58-1]
HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-IL-8 monoclonal antibody
(1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

FACS analysis of Anti-IL-8 Antibody [Clone 58-1]
Human peripheral blood mononuclear cells (PBMC) were stained with mouse
anti-IL-8 monoclonal antibody at 2 μg/ml (red) and without antibody control
(black).

ELISA titration of Anti-IL-8 Antibody [Clone 58-1]
Titration curve of anti-IL-8 antibody in ELISA. Red: IL-8; Black: BSA (negative
control).

Western blotting analysis of Anti-IL-8 Antibody [Clone 58-1]
Recombinant IL-8 protein (100 ng) was stained with mouse anti-IL-8
monoclonal antibody at 1:5000 dilution.

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Datasheet & Documents

Datasheet

MSDS

Manual

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