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Leadgene Leadgene
Anti-GIF Antibody [Clone 50-1]
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Catalog Number

LDG0044YA

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  • Overview

    Description

    Metallothioneins (MTs) is a group of low-molecular-weight, cysteine-rich, non-enzymatic proteins, is involved in storage, transportation and binding of metals, and plays a critical role in the protection against metal toxicity and oxidative stress. Metallothionein-3 (MT-3;also known as Growth Inhibitory Factor, GIF), a member of the MTs family, is mainly expressed in the central nervous system, can protect neuronal cells from oxidative stress via its anti-oxidant properties. MT-3 is abnormally under-expressed in the brains of Alzheimer's disease patients.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:100-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 50-1

    • Immunogen

      GIF

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      GIF

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-GIF Antibody [Clone 50-1] HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-GIF monoclonal antibody (1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-GIF Antibody [Clone 50-1]
    HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
    Triton X-100. Cells were stained with mouse anti-GIF monoclonal antibody
    (1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
    1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    FACS analysis of Anti-GIF Antibody [Clone 50-1] K562 cells were stained with mouse anti-GIF monoclonal antibody at 2 μg/ml (red) and without antibody control (black).

    FACS analysis of Anti-GIF Antibody [Clone 50-1]
    K562 cells were stained with mouse anti-GIF monoclonal antibody at 2 μg/ml
    (red) and without antibody control (black).

    ELISA titration of Anti-GIF Antibody [Clone 50-1] Titration curve of anti-GIF antibody in ELISA. Red: GIF; Black: BSA (negative control).

    ELISA titration of Anti-GIF Antibody [Clone 50-1]
    Titration curve of anti-GIF antibody in ELISA. Red: GIF; Black: BSA (negative
    control).

    Western blotting analysis of Anti-GIF Antibody [Clone 50-1] Recombinant proteins and mouse stomach lysates were stained with mouse anti-GIF monoclonal antibody at 1:5000 dilution. Lane 1: Recombinant GIF protein (100 ng). Lane 2: Mouse stomach lysate (30 μg). Lane 3: Human gastric juice (10 μL).

    Western blotting analysis of Anti-GIF Antibody [Clone 50-1]
    Recombinant proteins and mouse stomach lysates were stained with mouse
    anti-GIF monoclonal antibody at 1:5000 dilution. Lane 1: Recombinant GIF
    protein (100 ng). Lane 2: Mouse stomach lysate (30 μg). Lane 3: Human
    gastric juice (10 μL).

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  • Datasheet & Documents
    MSDS
    Manual
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