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Leadgene Leadgene
Anti-CXCL12 Antibody [Clone 69-2]
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Catalog Number

LDG0041YA

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  • Overview

    Description

    The CXC chemokine is a group of secreted proteins, attracts neutrophils and lymphocytes. CXC chemokine ligand 12 (CXCL12 or Stromal cell-derived factor 1, SDF-1), a member of the CXC chemokines, exists in three alternative splicing variants (α, β and γ). This chemokine plays a crucial role in embryogenesis, hematopoiesis, angiogenesis and inflammation. CXCL12 is the only chemokine ligand for CXCR4, this ligand-receptor pair is essential during embryogenesis, hematopoiesis, and immune system organization.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:200-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2a

      Clone Name

      clone 69-2

    • Immunogen

      CXCL12

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      CXCL12

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-CXCL12 Antibody [Clone 69-2] A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-CXCL12 monoclonal antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG- iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-CXCL12 Antibody [Clone 69-2]
    A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
    Triton X-100. Cells were stained with mouse anti-CXCL12 monoclonal

    antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-
    iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342

    (Blue).

    FACS analysis of Anti-CXCL12 Antibody [Clone 69-2] A549 cells were stained with mouse anti-CXCL12 monoclonal antibody at 2 μg/ml (red) and without antibody control (black).

    FACS analysis of Anti-CXCL12 Antibody [Clone 69-2]
    A549 cells were stained with mouse anti-CXCL12 monoclonal antibody at 2
    μg/ml (red) and without antibody control (black).

    ELISA titration of Anti-CXCL12 Antibody [Clone 69-2] Titration curve of anti-CXCL12 antibody in ELISA. Red: CXCL12; Black: BSA (negative control).

    ELISA titration of Anti-CXCL12 Antibody [Clone 69-2]
    Titration curve of anti-CXCL12 antibody in ELISA. Red: CXCL12; Black: BSA
    (negative control).

    Western blotting analysis of Anti-CXCL12 Antibody [Clone 69-2] HeLa cell lysates (30 μg) were stained with mouse anti-CXCL12 monoclonal antibody at 1:5000 dilution.

    Western blotting analysis of Anti-CXCL12 Antibody [Clone 69-2]
    HeLa cell lysates (30 μg) were stained with mouse anti-CXCL12 monoclonal
    antibody at 1:5000 dilution.

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  • Datasheet & Documents
    MSDS
    Manual
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