Swine IL-10, His Tag, E. coli

Catalog Number

LDG014PSE

Datasheet

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• Specifications

  • Species of Origin

    Swine

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 19.05 kDa.
    The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce proliferation in MC/9‑2 cells. The ED₅₀ for this effect is <5 ng/mL.

    Endotoxin level

    <0.01 EU per 1 µg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Interleukin 10 (IL-10) is a 17.54 kDa cytokine with 158 amino acid residues and is also known as human cytokine synthesis inhibitory factor (CSIF). IL-10 is an anti-inflammatory cytokine that is mainly secreted from monocytes, lymphocytes, and mast cells. IL-10 participates in the survival, proliferation, and antibody production of B cell via the JAK-STAT signaling pathway and suppress pro-inflammatory cytokine secretion by blocking the NF-κB signaling pathway.

    Synonyms

    Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

  • Uniprot ID

    #Q29055

    Sequence Note

    Ser19-Asn175

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant swine IL-10.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.