Leadgene Leadgene
Swine IL-1 Alpha, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)


      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.02 kDa.
      The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce D10.G4.1 cells proliferation. The ED₅₀ for this effect is <50 pg/mL

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      Interleukin 1-alpha (IL-1α), also named hematopoietin 1, is a 17.65 kDa cytokine with 159 amino acid residues. IL-1α is mainly expressed in macrophages, neutrophils, epithelial cells, and endothelial cells. When IL-1α binds to the IL-1 receptor, It mediates a wide variety of biological processes such as cell division, angiogenesis, production of interleukin-2, and cellular sodium ion homeostasis. In addition, IL-1α also plays a critical role in immune responses, such as fever and inflammation, and triggers tumor necrosis factor-alpha.


      Interleukin-1 alpha, IL-1 alpha

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant swine IL-1 alpha.

    SDS-PAGE analysis of recombinant swine IL-1 alpha.

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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